Order no.
BA128VS
Agent
Adenovirus Type 2 Hexon - Strain Adenoid 6
Culture system
Hep-2 cell culture
Preparation
Harvested infected cells are lysed, solvent treated to remove cellular components and ultracentrifuged to remove whole virus particles. Hexon antigen is purified from the centrifugation supernatant by ion exchange chromatography. The antigen is presented in 30 mM Bis-Tris buffer at pH 6.8 containing sodium chloride.
Inactivation
Whole infective virus particles are removed by ultracentrifugation and chromatography. Inactivity is confirmed by attempted growth under original culture conditions. Since no procedure can guarantee absolute sterility, the reagent should be handled with appropriate safety precautions.
Preservation
None
Protein concentration
Lot specific, specified in mg/mL. Determined by Bradford assay (Biorad) using BSA as standard or by UV absorption.
Analysis
By SDS-PAGE and ELISA
Storage
-20°C to -80°C. Avoid repeated freeze-thaw cycles.
Product Information for specific lots may vary.
