Order no.
BA1052VS
Agent
Herpes simplex virus Type 2 - Strain G
Culture system
Vero cell culture
Preparation
Virus extracted from infected cells is concentrated by ultracentrifugation and then partially purified by centrifugation through sucrose. The antigen is resuspended and homogenised. The resulting preparation consists of a high concentration of virus and viral components as well as some cellular material suspended in 0.1 M glycine buffer pH 9.5.
Inactivation
Beta-propiolactone treatment. Inactivity is confirmed by attempted growth under original culture conditions. Since no procedure can guarantee absolute sterility, the reagent should be handled with appropriate safety precautions.
Preservation
None
Protein concentration
Lot specific, specified in mg/mL. Determined by Bradford assay (Biorad) using BSA as standard.
Analysis
By ELISA
Storage
-20°C to -80°C. Avoid repeated freeze-thaw cycles.
Product Information for specific lots may vary.
