Order no.
BA103VS
Agent
Mumps virus
Culture system
Vero cell culture
Preparation
Infected cells and culture supernatant are harvested and concentrated by ultracentrifugation. Following sonication and clarification the suspension is again concentrated by centrifugation. The resulting antigen preparation consists of a high concentration of virus and viral components as well as some cellular material suspended in glycine buffer, pH 9.5.
Inactivation
Beta-propiolactone treatment. Inactivity is confirmed by attempted growth under original culture conditions. Since no procedure can guarantee absolute sterility, the reagent should be handled with appropriate safety precautions.
Preservation
None
Protein concentration
Lot specific, specified in mg/mL. Determined by Bradford assay (Biorad) using BSA as standard.
Analysis
By ELISA
Storage
-20°C to -80°C. Avoid repeated freeze-thaw cycles.
Product Information for specific lots may vary.
